Abstract:Dynamic conformational changes of proteins are crucial for their cellular functions. Here we present a unified refinement pipeline for flexible protein structures in both CryoEM and in situ CryoET. Using a Gaussian mixture model-based focused alignment procedure, we improve resolution of small domains in highly dynamic proteins and reveal intricate conformational changes. The method corrects the per-subunit motion of TRPV1 and captures the rotary dynamics of ATP synthase within mitochondria.
From: Muyuan Chen [view email]
[v1]
Thu, 28 May 2026 19:55:17 UTC (5,915 KB)